|
| Status |
Public on May 27, 2016 |
| Title |
MCF-10A_Control, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-10A transfected with a control vector
|
| Organism |
Homo sapiens |
| Characteristics |
strain: MCF-10A mammary epithelial cell line
|
| Treatment protocol |
SNAI1 or a vector control was introduced into MCF-10A cells, respectively.
|
| Growth protocol |
MCF-10A mammary epithelial cells were cultured according to the manufacturer's instructions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using 1 Poly-A RNA Control Kit (Affymetrix, P/N 900433) and MessageAmp™ Premier RNA Amplification Kit (Ambion,＃1792) from 1ug total RNA, according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5ug of cRNA were hybridized for 16h at 45℃ on GeneChip HG-U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix ® Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix ® GeneChip® Scanner 3000.
|
| Description |
gene expression data from MCF-10A transfected with a vector control
|
| Data processing |
The data were analyzed with Affymetrix® GeneChip® Command Console™ 1.1 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
May 26, 2016 |
| Last update date |
May 27, 2016 |
| Contact name |
Zhihua Liu |
| E-mail(s) |
liuzh@cicams.ac.cn
|
| Phone |
8610-67723789
|
| Organization name |
National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
|
| Street address |
NO.17 Nanli, Panjiayuan Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100021 |
| Country |
China |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE81929 |
SNAI1 overexpression effect on MCF-10A mammary epithelial cell line (mRNA) |
| GSE81931 |
SNAI1 overexpression effect on MCF-10A mammary epithelial cell line |
|
