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Sample GSM2177876 Query DataSets for GSM2177876
Status Public on May 27, 2016
Title MCF-10A_Control, biological rep3
Sample type RNA
 
Source name MCF-10A transfected with a control vector
Organism Homo sapiens
Characteristics strain: MCF-10A mammary epithelial cell line
Treatment protocol SNAI1 or a vector control was introduced into MCF-10A cells, respectively.
Growth protocol MCF-10A mammary epithelial cells were cultured according to the manufacturer's instructions.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared using 1 Poly-A RNA Control Kit (Affymetrix, P/N 900433) and MessageAmp™ Premier RNA Amplification Kit (Ambion,#1792) from 1ug total RNA, according to the manufacturer's instructions.
 
Hybridization protocol Following fragmentation, 5ug of cRNA were hybridized for 16h at 45℃ on GeneChip HG-U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix ® Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix ® GeneChip® Scanner 3000.
Description gene expression data from MCF-10A transfected with a vector control
Data processing The data were analyzed with Affymetrix® GeneChip® Command Console™ 1.1 using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date May 26, 2016
Last update date May 27, 2016
Contact name Zhihua Liu
E-mail(s) liuzh@cicams.ac.cn
Phone 8610-67723789
Organization name National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
Street address NO.17 Nanli, Panjiayuan Road, Chaoyang District
City Beijing
ZIP/Postal code 100021
Country China
 
Platform ID GPL570
Series (2)
GSE81929 SNAI1 overexpression effect on MCF-10A mammary epithelial cell line (mRNA)
GSE81931 SNAI1 overexpression effect on MCF-10A mammary epithelial cell line

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 1595.936644
1053_at 395.9639169
121_at 254.4523089
1316_at 86.47086893
1487_at 476.2336476
1494_f_at 53.53392027
1552256_a_at 951.1595186
1552257_a_at 768.3553745
1552258_at 181.1520536
1552263_at 169.0528384
1552264_a_at 185.1082235
1552274_at 88.35895158
1552275_s_at 64.49987391
1552277_a_at 924.6138409
1552279_a_at 218.6375272
1552286_at 72.63752717
1552287_s_at 422.4743483
1552291_at 232.6233064
1552295_a_at 297.8956227
1552309_a_at 39.58046852

Total number of rows: 21772

Table truncated, full table size 484 Kbytes.




Supplementary file Size Download File type/resource
GSM2177876_MCF-10A_C3_HG-U133_Plus_2_.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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