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Status |
Public on May 27, 2016 |
Title |
MCF-10A_Control, biological rep3 |
Sample type |
RNA |
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Source name |
MCF-10A transfected with a control vector
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Organism |
Homo sapiens |
Characteristics |
strain: MCF-10A mammary epithelial cell line
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Treatment protocol |
SNAI1 or a vector control was introduced into MCF-10A cells, respectively.
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Growth protocol |
MCF-10A mammary epithelial cells were cultured according to the manufacturer's instructions.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared using 1 Poly-A RNA Control Kit (Affymetrix, P/N 900433) and MessageAmp™ Premier RNA Amplification Kit (Ambion,#1792) from 1ug total RNA, according to the manufacturer's instructions.
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Hybridization protocol |
Following fragmentation, 5ug of cRNA were hybridized for 16h at 45℃ on GeneChip HG-U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix ® Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix ® GeneChip® Scanner 3000.
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Description |
gene expression data from MCF-10A transfected with a vector control
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Data processing |
The data were analyzed with Affymetrix® GeneChip® Command Console™ 1.1 using Affymetrix default analysis settings and global scaling as normalization method.
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Submission date |
May 26, 2016 |
Last update date |
May 27, 2016 |
Contact name |
Zhihua Liu |
E-mail(s) |
liuzh@cicams.ac.cn
|
Phone |
8610-67723789
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Organization name |
National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
|
Street address |
NO.17 Nanli, Panjiayuan Road, Chaoyang District
|
City |
Beijing |
ZIP/Postal code |
100021 |
Country |
China |
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|
Platform ID |
GPL570 |
Series (2) |
GSE81929 |
SNAI1 overexpression effect on MCF-10A mammary epithelial cell line (mRNA) |
GSE81931 |
SNAI1 overexpression effect on MCF-10A mammary epithelial cell line |
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