|
| Status |
Public on Jan 01, 2017 |
| Title |
HT15 |
| Sample type |
RNA |
| |
|
| Source name |
tonsils
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: tonsils
disease state: healthy tonsils
hpv status: healthy tonsils
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion RecoverAll from Formalin Fixed Paraffin Embedded material
|
| Label |
n/a
|
| Label protocol |
RNA was not labeled; instead, RNA (33 ng/ul) was pre-processed following the NanoString manufacturer's protocol by the Ohio State Microarray Core Facility
|
| |
|
| Hybridization protocol |
RNA was hybridized to capture probes and detection probes using the NanoString nCounter system according to the manufacturer's protocol. Detection probes were provided by the manufacturer and were labeled with a proprietary fluorescent barcode system.
|
| Scan protocol |
Scanning was conducted using the NanoString confocal microscope scanner/Digital Analyzer according to manufacturer's instructions.
|
| Data processing |
The analysis of raw data was performed with nSlover Analysis Software provided by NanoString Technologies. Data were collected as NanoString RCC files and provided as an Excel spreadsheet. The data were normalizeed to the geometric mean of the 90 most highly expressed miRNAs.
|
| |
|
| Submission date |
May 31, 2016 |
| Last update date |
Jan 01, 2017 |
| Contact name |
Luciano Cascione |
| E-mail(s) |
luciano.cascione@ior.usi.ch
|
| Organization name |
IOR
|
| Department |
Lymphoma & Genomics Research Program
|
| Street address |
Via Chiesa 5
|
| City |
Bellinzona |
| State/province |
Ticino |
| ZIP/Postal code |
6500 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL21968 |
| Series (1) |
| GSE82064 |
MicroRNA-expression in tonsillar and base of tongue cancer related to HPV and clinical outcome |
|
