|
| Status |
Public on Jun 02, 2016 |
| Title |
8R CA1 total RNA, rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
8R CA1 total RNA
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: male
strain: Long Evans
stress: 10 min ischemia and 8 hours reperfusion
tissue: brain
region: CA1
molecule type: total RNA
|
| Treatment protocol |
complete normothermic global brain ischemia was induced by 2 vessel carotid artery occlusion and hypovoluemic hypotension to MAP = 45 mm Hg
|
| Growth protocol |
standard rat laboratory housing
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Fragmented, biotin-labeled cDNA was hybridized for 17 hr at 45oC to GeneChip Rat Gene 2.0 ST Arrays
|
| Scan protocol |
Fluorescence intensities were determined using a GCS 3000 7G high-resolution confocal laser scanner.
|
| Description |
8hR1T4
gene expression data from CA1 after 10 min ischemia and 8 hours reperfusion
|
| Data processing |
Background correction by the PM-CGBG method and probe set signal summarization using the PLIER algorithm was performed in Affymetrix Expression Console. Quantile normalization, filtering, and class comparisons to identify probe sets exhibiting statistically significant differential expression were performed using BRB-ArrayTools
|
| |
|
| Submission date |
Jun 02, 2016 |
| Last update date |
Jun 02, 2016 |
| Contact name |
Donald James DeGracia |
| E-mail(s) |
ddegraci@med.wayne.edu
|
| Phone |
313-577-6745
|
| Organization name |
Wayne State University
|
| Department |
Physiology
|
| Lab |
4116 Scott Hall
|
| Street address |
540 E. Canfield
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE82146 |
Polysomal and total RNA in controls and after 10 min global brain ischemia and 8 hours of reperfusion |
|
