|
| Status |
Public on Jun 03, 2016 |
| Title |
normal control-14 [NC14] |
| Sample type |
genomic |
| |
|
| Source name |
peripheral blood mononuclear cell
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
Sex: Male
age: 30y
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolation from whole blood cells using genomic DNA extraction kits (Life Technologies, Gaithersburg, MD).
|
| Label |
biotin
|
| Label protocol |
Genomic DNA (1μg) was bisulfite converted using EZ DNA Methylation kit (Zymo Research Corp, Orange, CA. Cat. No. D5004), and utilizing a cyclic denaturation step during the conversion reaction. 4ul of bisulfite-converted DNA were used for hybridization on Infinium HumanMethylation 450 BeadChip.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 14
|
| Data processing |
Expression values were extracted, filtered and normalized using an R package (RnBeads). The normalized methylation data were analyzed by SAM (significance analysis of microarrays, Stanford University)
|
| |
|
| Submission date |
Jun 03, 2016 |
| Last update date |
Jun 03, 2016 |
| Contact name |
yun lian |
| E-mail(s) |
yun.lian@utsouthwestern.edu
|
| Organization name |
UT Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE82218 |
Whole genome transcription and DNA methylation analysis of peripheral blood mononuclear cells identified aberrant gene regulation pathways in systemic lupus erythematosus [metylation] |
| GSE82221 |
Whole genome transcription and DNA methylation analysis of peripheral blood mononuclear cells identified aberrant gene regulation pathways in systemic lupus erythematosus |
|
