|
| Status |
Public on Jun 04, 2016 |
| Title |
3-IFN-γ -siC-GW |
| Sample type |
RNA |
| |
|
| Source name |
Gene expression data from IFN-γ- and GW-treated astrocytes that were control siRNA transfected
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
age: 1 day-old
tissue: cerebral cortex
cell type: astrocytes
|
| Treatment protocol |
Astrocytes transfect with control siRNA or SHP siRNA and treated with IFN-γ in the presence or absence of GW for 3 h
|
| Growth protocol |
Primary astrocytes were prepared by trypsinization. To deplete microglia and meningeal cells, astrocytes were incubated with serum-free MEM for 2 days before use
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was then fragmented and biotin-labeled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip WT Terminal labeling kit
|
| |
|
| Hybridization protocol |
Approximately 5.5 μg of labeled DNA target was hybridized to the Affymetrix GeneChip Array at 45°C for 16hour
|
| Scan protocol |
Hybridized arrays were washed and stained on a GeneChip Fluidics Station 450 and scanned on a GCS3000 Scanner (Affymetrix).
|
| Description |
control siRNA, IFN-γ, GW
|
| Data processing |
Array data export processing and analysis was performed using Affymetrix® GeneChip Command Console® Software (AGCC)
|
| |
|
| Submission date |
Jun 03, 2016 |
| Last update date |
Jun 04, 2016 |
| Contact name |
jeehoon lee |
| E-mail(s) |
if7979@hotmail.com
|
| Organization name |
Ajou university, School of Medicine
|
| Department |
Pharmacology
|
| Street address |
San5, Woncheondong
|
| City |
Suwon |
| State/province |
Gyoungido |
| ZIP/Postal code |
442-721 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE82247 |
Expression data from SHP specific siRNA or nonspecefic siRNA transfected rat astrocytes [extended study] |
|
