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| Status |
Public on Dec 01, 2017 |
| Title |
GFP-Serum rep 1 |
| Sample type |
SRA |
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| Source name |
U2OS cell line
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| Organism |
Homo sapiens |
| Characteristics |
genotype/variation: GFP
condition: Serum
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from U2OS cells overexpressing actin mutant G13R and those expressing GFP as control, at both normal-growth and serum-stimulation conditions. Poly(A)+ mRNAs were enriched using Dynabeads oligo (dT) beads (NEB). RT reactions and purification of cDNA templates were performed following the RNA-seq sample preparation protocol from Illumina. Each cDNA sample was sequenced on an IlluminaHiseq 2500 system.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Data processing |
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence
Aligned using the TopHat (v2.0.13) with the Ensembl Drosophila_melanogaster.BDGP5.78.gtf as a reference.
The transcription analysis was performed using Cufflinks (v2.2.1)
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample ...
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| Submission date |
Jun 07, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ruifeng Li |
| E-mail(s) |
lirf@pku.edu.cn
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| Organization name |
Peking University
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| Street address |
Haidian
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| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platform ID |
GPL16791 |
| Series (1) |
| GSE82320 |
Nuclear Actin Regulates Inducible Transcription by Enhancing RNA Polymerase II Clustering |
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| Relations |
| BioSample |
SAMN05212066 |
| SRA |
SRX1826069 |
| Supplementary data files not provided |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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