|
Status |
Public on Feb 03, 2017 |
Title |
TG39_DMSO_rep1 |
Sample type |
SRA |
|
|
Source name |
thymoma primary T-ALL culture
|
Organism |
Mus musculus |
Characteristics |
type: T-ALL treatment: DMSO
|
Treatment protocol |
Cells were treated with DMSO or 100nM GSK2879552 for 24h
|
Growth protocol |
mouse T-ALL cells were seeded at 0.5 x 10^6 cells/ml. Cells were cultured in RPMI 1640 medium supplemented with 10% FBS, 100 U/ml penicillin, 100 µg/ml streptomycin and 2 mM L-glutamine at 37°C with 5% CO2.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using the RNeasy Plus mini kit (Qiagen). polyA Illumina Stranded Sample prep
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 1500 |
|
|
Description |
RNA-sequencing of P53/R26-Zeb2tg/tg TG39 cells treated with DMSO for 24h
|
Data processing |
base calling with Casava 1.8 read alignment and gene quantification on ENSG were performed with STAR v2.4.2a DESeq2 was used for differential expression analisis using a multifactorial design Genome_build: GRCm38 Supplementary_files_format_and_content: STAR raw count data, tdt
|
|
|
Submission date |
Jun 08, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Sofie Peirs |
E-mail(s) |
sofie.peirs@ugent.be
|
Organization name |
Ghent University
|
Street address |
De Pintelaan 185
|
City |
Gent |
ZIP/Postal code |
9000 |
Country |
Belgium |
|
|
Platform ID |
GPL18480 |
Series (2) |
GSE83127 |
Effect of 24h treatment with 100nM GSK2879552 on the murine T-ALL cell with and without Zeb2 overexpression |
GSE83128 |
Oncogenic ZEB2 activation drives sensitivity towards LSD1 inhibition in T-cell acute lymphoblastic leukemia |
|
Relations |
BioSample |
SAMN05215798 |
SRA |
SRX1830803 |