|
| Status |
Public on Feb 03, 2017 |
| Title |
TG50_LSD1i_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
thymoma primary T-ALL culture
|
| Organism |
Mus musculus |
| Characteristics |
type: T-ALL
treatment: GSK2879552
|
| Treatment protocol |
Cells were treated with DMSO or 100nM GSK2879552 for 24h
|
| Growth protocol |
mouse T-ALL cells were seeded at 0.5 x 10^6 cells/ml. Cells were cultured in RPMI 1640 medium supplemented with 10% FBS, 100 U/ml penicillin, 100 µg/ml streptomycin and 2 mM L-glutamine at 37°C with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy Plus mini kit (Qiagen).
polyA Illumina Stranded Sample prep
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Description |
RNA-sequencing of P53/R26-Zeb2tg/tg TG50 cells treated with 100nM GSK2879552 for 24h
|
| Data processing |
base calling with Casava 1.8
read alignment and gene quantification on ENSG were performed with STAR v2.4.2a
DESeq2 was used for differential expression analisis using a multifactorial design
Genome_build: GRCm38
Supplementary_files_format_and_content: STAR raw count data, tdt
|
| |
|
| Submission date |
Jun 08, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Sofie Peirs |
| E-mail(s) |
sofie.peirs@ugent.be
|
| Organization name |
Ghent University
|
| Street address |
De Pintelaan 185
|
| City |
Gent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL18480 |
| Series (2) |
| GSE83127 |
Effect of 24h treatment with 100nM GSK2879552 on the murine T-ALL cell with and without Zeb2 overexpression |
| GSE83128 |
Oncogenic ZEB2 activation drives sensitivity towards LSD1 inhibition in T-cell acute lymphoblastic leukemia |
|
| Relations |
| BioSample |
SAMN05215817 |
| SRA |
SRX1830812 |
