|
| Status |
Public on Nov 15, 2016 |
| Title |
N_LEF1_KD_1 |
| Sample type |
RNA |
| |
|
| Source name |
CD8 Naive LEF1 KD 1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Blood
treatment: CD8 Naive LEF1 KD
|
| Growth protocol |
Naive CD8+ T cells were isolated from PBMCs by magnetic bead separation using a Naive CD8+ T cell isolation Kit (Myltenyi Biotec)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from naive CD8+ T cells using RNAqueous-Micro kit (Ambion)
|
| Label |
Cy3
|
| Label protocol |
TargetAmp™ Nano-g™ Biotin-aRNA Labeling Kit for the Illumina® System (Epicentre)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with lumi in R. After background correction with GenomeStudio and log2 transformation, probes with a detection p-value greater than 0.01 for all samples were discarded, using the detectionCall function in the lumi R package. That is, probes with detection p-value smaller than 0.01 were chosen for the normalization.
|
| |
|
| Submission date |
Jun 09, 2016 |
| Last update date |
Nov 15, 2016 |
| Contact name |
Jose Luis Lavin |
| E-mail(s) |
joluito@gmail.com
|
| Organization name |
CIC bioGUNE
|
| Street address |
Parque Tecnológico de Bizkaia Building 502, Floor 0
|
| City |
Derio |
| State/province |
Bizkaia |
| ZIP/Postal code |
48160 |
| Country |
Spain |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE83158 |
Epigenetic regulation of the transcriptional program in memory and terminally differentiated CD8+ T cells [HCAFIS_12_Gene_Expression] |
| GSE83159 |
Epigenetic regulation of the transcriptional program in memory and terminally differentiated CD8+ T cells |
|
