|
| Status |
Public on Jun 10, 2016 |
| Title |
fvb-10wk-miRNAseq-rep3 |
| Sample type |
SRA |
| |
|
| Source name |
fvb-mammary-gland
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB
genotype/variation: control
Stage: control
time: 10wk
tissue: mammary gland
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mammary tissue was snap-frozen, later total RNA was extracted with miRNeasy (Qiagen).
Total RNA was used to create small RNA libraries using Illumina trueseq small RNA KIT Version 1.
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
PyMT_22.AC1BR7ACXX.lane_2_P0_I22.mm9
|
| Data processing |
Sequences ranging from 17 - 27 bases (the size of mature microRNAs) are aligned to genomic and RefSeq libraries using Bowtie.
Sequences that align to annotated regions of the genome are compared with known microRNAs in miRBase. A hit is defined as an aligned sequence that overlaps at least 50% with coordinates of a miRBase-defined microRNA.
Genome_build: mm9
|
| |
|
| Submission date |
Jun 09, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
ying cai |
| Organization name |
Albert Einstein College of Medicine
|
| Street address |
1300 morris park ave
|
| City |
bronx |
| State/province |
New York |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE83189 |
PyMT 4 stages tumor progression microRNA sequencing |
|
| Relations |
| BioSample |
SAMN05223353 |
| SRA |
SRX1835973 |
