|
| Status |
Public on Dec 31, 2016 |
| Title |
Male treatment, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Liver of male mice received 100nM CdCl2
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
gender: Male
exposure: Cadmium Chloride 100nM
|
| Treatment protocol |
Female mice were exposed by water or 100nM cadmium chloride in water continually from 1 week before copulation. After weaning, the offsprings were seperately housed with the supply of water or 100nM cadmium chloride in water continually untill 30 weeks of age, when they were sacrificed and the liver were sampled.
|
| Growth protocol |
Female mice were caged individually during mating, pregnancy and lactation. And the offsprings were separately housed after weaning.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Takara RNAiso Plus Kit (Cat#9109, Takara) following the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotin labeled cRNA were obtained by using GeneChip 3' IVT PLUS Reagent Kit (Cat#902416, Affymetrix) followed the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Array hybridization and wash was performed using GeneChip® Hybridization, Wash and Stain Kit (Cat#900720, Affymetrix) in Hybridization Oven 645 and Fluidics Station 450 followed the manufacturer’s instructions.
|
| Scan protocol |
Slides were scanned by GeneChip® Scanner 3000.
|
| Description |
Gene expression data from liver of male mice received CdCl2
|
| Data processing |
Raw data were normalized by MAS 5.0 algorithm, Gene Spring Software12.6.1.
|
| |
|
| Submission date |
Jun 14, 2016 |
| Last update date |
Dec 31, 2016 |
| Contact name |
Qian Ba |
| Organization name |
Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
|
| Street address |
320 Yueyang Rd
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE83326 |
Hepatic gene expression data from cadmium-exposed mice |
|
