Total leukocytes were isolated from 9 ml whole blood and its total RNA stabilized and extracted using the LeukoLOCK™ Total RNA Isolation System (Ambion, Austin, TX)
Label
Cy3
Label protocol
cRNA labelling using Cy3 was performed using the Illumina TotalPrep RNA Amplification kit (Ambion, Austin, TX) following the manufacturer’s instructions.
Hybridization protocol
Illumina hybridization recommencded protocol was followed.
Scan protocol
The BeadChips were imaged using Illumina’s BeadArray Reader
Description
none
Data processing
The raw intensities were extracted using the Gene Expression Module in Illumina’s BeadStudio software
