|
| Status |
Public on Sep 01, 2016 |
| Title |
245B 50 Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
EndoC-betaH1 cell line (human beta cell line)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: EndoC-betaH1
replicate: Replicate 2
lentivirus: HI-LNC85sh2
|
| Treatment protocol |
Five non-targeting and four amiRNAs targeting each of 5 TF and 25 lncRNAs were designed using
|
| Growth protocol |
EndoC-betaH1 were maintained as previously described (Ravassard et al.,
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Fragmented and labeled ss-cDNA was prepared according to Affymetrix WT PLUS Reagent Kit user guide
|
| |
|
| Hybridization protocol |
Following fragmentation and terminal labeling, 5.2ug of ss-cDNA were hybridized for 16hr at 45C on GeneChip Human Transcriptome Array 2.0. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneCHip Scanner 3000 System.
|
| Data processing |
The data were analyzed with Expression Console Software using RMA analysis.
|
| |
|
| Submission date |
Jun 22, 2016 |
| Last update date |
Sep 01, 2016 |
| Contact name |
ildem akerman |
| Organization name |
University of Birmingham
|
| Department |
IMSR
|
| Lab |
Akerman Lab
|
| Street address |
BR Tower, 3rd Floor, Room 333, Edgbaston,
|
| City |
birmingham |
| ZIP/Postal code |
B15 2TT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE83619 |
HI-LNC RNA knockdown in EndoC-betaH1 cell using lentiviral ami-aRNAs |
|
