|
| Status |
Public on Jun 24, 2016 |
| Title |
20 SP6-embryonic skin-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
20 SP6-embryonic skin
|
| Organism |
Xenopus laevis |
| Characteristics |
developmental stage: embryonic stage 24
treated with: 100mM SP600125 (from stage 14 to stage 24)
tissue: whole embryonic skin
|
| Treatment protocol |
100mM SP600125 (Jnk inhibitor) treatment from stage 14 to stage 24
|
| Growth protocol |
embryos are grown in 0,1x MBS at 15 °C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of Total RNA was performed according the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
biotin
|
| |
|
| Hybridization protocol |
following the manufacturer instructions with GeneChip 3'IVT PLUS Reagent Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 +7G
|
| Description |
sp6_1
|
| Data processing |
All microarray data preprocessing and analysis was done using R. Affy CEL files were imported using the affy package. Expression intensities were summarized, normalized, and transformed using Robust Multiarray Average algorithm. Please note that all the genes that did not map to an Entrez ID were excluded, resulting in 16,755 data rows.
|
| |
|
| Submission date |
Jun 23, 2016 |
| Last update date |
Jun 24, 2016 |
| Contact name |
Amanda J Dickinson |
| E-mail(s) |
ajdickinson@vcu.edu
|
| Organization name |
Virginia Commonwealth University
|
| Department |
Biology
|
| Lab |
Dickinson
|
| Street address |
1000 W Cary street
|
| City |
Richmond |
| State/province |
Virginia |
| ZIP/Postal code |
23284 |
| Country |
USA |
| |
|
| Platform ID |
GPL10756 |
| Series (1) |
| GSE83672 |
Expression data from Xenopus laevis embryonic epidermis depleted for Jnk signalling |
|
