|
| Status |
Public on Nov 18, 2016 |
| Title |
Candida albicans-systemic mouse infection-liver-8h_rep1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
common reference, log-phase growth in YPD, 37°C
|
| Organism |
Candida albicans |
| Characteristics |
control type: common reference
|
| Treatment protocol |
tail vein infection of mice
|
| Growth protocol |
stationary growth, YPD, 30°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Phenol:Chloroform:Isoamylalcohol extraction method
|
| Label |
Cy3
|
| Label protocol |
Low RNA Input Fluorescent Linear amplification Kit, Agilent Technologies, Santa Clara, CA, USA
|
| |
|
| Channel 2 |
| Source name |
liver isolation, 8h p.i.
|
| Organism |
Candida albicans |
| Characteristics |
candida albicans strain: SC5314
host mouse tissue: liver
hours post infection: 8
|
| Treatment protocol |
tail vein infection of mice
|
| Growth protocol |
stationary growth, YPD, 30°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Phenol:Chloroform:Isoamylalcohol extraction method
|
| Label |
Cy5
|
| Label protocol |
Low RNA Input Fluorescent Linear amplification Kit, Agilent Technologies, Santa Clara, CA, USA
|
| |
|
| |
| Hybridization protocol |
co-hybridized at a ratio of 1:1 with common reference
|
| Scan protocol |
428TM array scanner, Affymetrix
|
| Data processing |
Using R 2.15.2 with library limma. Printtiploess for within-array normalisation. Quantile normalisation (Manormbet function) between different arrays.s
|
| |
|
| Submission date |
Jun 23, 2016 |
| Last update date |
Nov 18, 2016 |
| Contact name |
Ilse Denise Jacobsen |
| E-mail(s) |
ilse.jacobsen@hki-jena.de
|
| Phone |
+49 3641 5321223
|
| Organization name |
Leibniz Institute for Natural Product Research and Infection Biology
|
| Lab |
Microbial Immunology
|
| Street address |
Beutenbergstr. 11a
|
| City |
Jena |
| ZIP/Postal code |
07745 |
| Country |
Germany |
| |
|
| Platform ID |
GPL22078 |
| Series (2) |
| GSE83679 |
Combined transcriptional profiling during systemic candidiasis reveals organ-specific host-pathogen interactions [C. albicans] |
| GSE83682 |
Combined transcriptional profiling during systemic candidiasis reveals organ-specific host-pathogen interactions |
|
