|
| Status |
Public on Nov 01, 2017 |
| Title |
SD-RSA_20797 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Recurrent miscarriage couples
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
clinical indication: recurrent miscarriage couples
|
| Treatment protocol |
Samples were untreated
|
| Growth protocol |
Uncultured samples of chorionic villus sampling, amniotic fluid, placental tissue and blood were used for direct DNA extraction
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA extraction done by Qiagen DNeasy® Blood & Tissue Kit following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Procedure for DNA fragmentation and labelling: 300 ng of Genomic DNA samples were heat fragmented. The fragmented DNA samples were labeled with Cy5 and the corresponding fragmented reference controls were labeled Cy3 using Agilent SureTag DNA Labeling Kit.
|
| |
|
| Channel 2 |
| Source name |
healthy Chinese adult
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
clinical indication: healthy
|
| Treatment protocol |
Samples were untreated
|
| Growth protocol |
Uncultured samples of chorionic villus sampling, amniotic fluid, placental tissue and blood were used for direct DNA extraction
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA extraction done by Qiagen DNeasy® Blood & Tissue Kit following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Procedure for DNA fragmentation and labelling: 300 ng of Genomic DNA samples were heat fragmented. The fragmented DNA samples were labeled with Cy5 and the corresponding fragmented reference controls were labeled Cy3 using Agilent SureTag DNA Labeling Kit.
|
| |
|
| |
| Hybridization protocol |
After clean up and QC check, Cy3 and Cy5 labeled DNA samples were mixed together with Cot-1 DNA, blocking agent and hybridization buffer using Agilent Oligo aCGH/ChIP-on-Chip Hybridization kit according to manufacturer's protocol. Samples were hybridized on array for 24 hours at 65oC. After hybridization, slides were washed sequential
|
| Scan protocol |
Probe is designed based on hg19. Scanned on an Agilent G2505C scanner.
|
| Data processing |
Agilent Feature Extraction Software (v 11.5.1.1) was used for background subtraction
|
| |
|
| Submission date |
Jul 01, 2016 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Zirui DONG |
| E-mail(s) |
elvisdong@link.cuhk.edu.hk
|
| Organization name |
The Chinese University of Hong Kong
|
| Department |
Dept. of Obstetrics and Gynaecology
|
| Street address |
Shatin, N.T.
|
| City |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
China |
| |
|
| Platform ID |
GPL20899 |
| Series (1) |
| GSE83941 |
Chromosomal microarray analysis for validation of the WGS-based CNV detection results in recurrent miscarriage couples |
|
