|
| Status |
Public on Jul 01, 2019 |
| Title |
YSL_PE-089 |
| Sample type |
RNA |
| |
|
| Source name |
Lung cancer cells, was established from taiwan lung adenocarcinoma patients
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Lung
tumor type: lung adenocacinoma
|
| Treatment protocol |
All 16 cells were parental and non-treated.
|
| Growth protocol |
All human lung adenocarcinoma cell lines were grown in RPMI-1640 media supplemented with 10% FBS. All cell lines were incubated at 37°C in a humidified 20% O2/5% CO2 environment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
cRNA were prepared with the labeling Cy3 (Illumina) for Illumina Expression BeadChip
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Parental, non-treated cells
|
| Data processing |
To focus on the functional miRNAs we only analyzed the miRbase annotated miRNAs (858 out of 1146 miRNAs). The data were treated by log2 transformation and quantile normalization using JMP software.
|
| |
|
| Submission date |
Jul 01, 2016 |
| Last update date |
Jul 01, 2019 |
| Contact name |
Sung-Liang Yu |
| E-mail(s) |
slyu@ntu.edu.tw
|
| Phone |
886-2-23958341
|
| Organization name |
National Taiwan University
|
| Department |
Clinical Laboratory Sciences and Medical Biotechnology
|
| Lab |
Microarray Core Facility
|
| Street address |
Jen Ai Road Section1
|
| City |
Taipei |
| ZIP/Postal code |
100 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL8179 |
| Series (2) |
| GSE83949 |
The gene regulation of miR-10a* in lung cancer (microRNA) |
| GSE83952 |
The gene regulation of miR-10a* in lung cancer |
|
