|
Status |
Public on Dec 12, 2016 |
Title |
PDF-EGFP 29C-3 |
Sample type |
SRA |
|
|
Source name |
PDF-EGFP 29C
|
Organism |
Drosophila melanogaster |
Characteristics |
strain: PDF-GAL4;UAS-EGFP age: young temperature: 29C time (min): 60 Sex: pooled male and female cell type: PDF+ neurons
|
Treatment protocol |
Flies were reared at 18C and temperature shift to 29C for stimulation for 60min.
|
Growth protocol |
Drosophila were reared on standard cornmeal/agar medium supplemented with yeast under 12 hour LD cycles at 25 °C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Brains were dissected for neuron sorting and cells were stored in lysis buffer (Ambion, Dynabead mRNA extraction kit) at -80C. refer to Abruzzi et al., 2014
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
NextSeq 550 |
|
|
Description |
Sample 85
|
Data processing |
cl2fastq v2.16.0.10 is used for base-calling tophat for alignment cufflinks for quantification Genome_build: dm3 Supplementary_files_format_and_content: [fpkm_tracking] files generated by Cufflinks include FPKM values for each gene
|
|
|
Submission date |
Jul 01, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Xiao Chen |
E-mail(s) |
chxiao@brandeis.edu
|
Organization name |
Brandeis University
|
Department |
Biology
|
Lab |
Rosbash Lab
|
Street address |
415 South St
|
City |
Waltham |
State/province |
MA |
ZIP/Postal code |
02451 |
Country |
USA |
|
|
Platform ID |
GPL22106 |
Series (2) |
GSE83969 |
Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila [DA_PDF] |
GSE83976 |
Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila |
|
Relations |
Reanalyzed by |
GSM3281590 |
BioSample |
SAMN05346378 |
SRA |
SRX1892913 |