|
| Status |
Public on Dec 12, 2016 |
| Title |
0' NUN |
| Sample type |
SRA |
| |
|
| Source name |
0' NUN
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Elav-GAL4;UAS-ChR2-XXL
stimulation: BLUE LED
time (min): 0
age: young
Sex: pooled male and female
tissue: heads
|
| Treatment protocol |
Flies were on ATR-containing food for 1 day in dark. 30-second LED stimulation was applied the next day.
|
| Growth protocol |
Drosophila were reared on standard cornmeal/agar medium supplemented with yeast under 12 hour LD cycles at 25 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Brains were dissected and RNA immediately extracted with TRIzol.
Illumina mRNA library contruction protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Description |
Sample 54
nascent RNA
|
| Data processing |
cl2fastq v2.16.0.10 is used for base-calling
tophat for alignment
cufflinks for quantification
Genome_build: dm3
Supplementary_files_format_and_content: [fpkm_tracking] files generated by Cufflinks include FPKM values for each gene
|
| |
|
| Submission date |
Jul 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Xiao Chen |
| E-mail(s) |
chxiao@brandeis.edu
|
| Organization name |
Brandeis University
|
| Department |
Biology
|
| Lab |
Rosbash Lab
|
| Street address |
415 South St
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
02451 |
| Country |
USA |
| |
|
| Platform ID |
GPL22106 |
| Series (2) |
| GSE83970 |
Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila [Elav_ChR2] |
| GSE83976 |
Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila |
|
| Relations |
| Reanalyzed by |
GSM3281611 |
| BioSample |
SAMN05341947 |
| SRA |
SRX1892935 |
