|
| Status |
Public on Jan 01, 2017 |
| Title |
Rspo1 1 |
| Sample type |
RNA |
| |
|
| Source name |
Rpos1 null_primary myoblasts
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57bl6N/sv129
genotype/variation: Rpos1-null
tissue: limb muscles
cell type: Primary myoblasts
|
| Treatment protocol |
Differentiating myocytes harvested fater 24 hrs in differentiating conditions.
|
| Growth protocol |
Cells were grown in Ham F10 media with 20%FBS and 2ng/ml bFGF for 5 passage. Differentiation was induced by serum removal and culture in DMEM with 2% HS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse Gene from Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
|
| Data processing |
the data were RMA normalized and analysed with Partek Genomic Suit
|
| |
|
| Submission date |
Jul 05, 2016 |
| Last update date |
Jan 01, 2017 |
| Contact name |
Fabien Le Grand |
| E-mail(s) |
fabien.le-grand@cnrs.fr
|
| Phone |
0033144412436
|
| Organization name |
Institut Neuromyogene
|
| Department |
Melis
|
| Street address |
8 avenue Rockefeller
|
| City |
Lyon |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL20710 |
| Series (1) |
| GSE84016 |
Expression data from Rspo1-null differentiating primary myoblasts |
|
