|
| Status |
Public on Jul 06, 2016 |
| Title |
Horse_1_CD14_Mo |
| Sample type |
RNA |
| |
|
| Source name |
equine myeloid cells
|
| Organism |
Equus caballus |
| Characteristics |
serum used: no serum
|
| Treatment protocol |
One group of
|
| Growth protocol |
Differentiation into eqMoDC was induced by addition of 25 ng/ml equine GM-CSF and 10 ng/ml equine IL-4 for 3 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNAqueous micro Kit (Life Technologies). RNA quality was assessed with the RNA 6000 Pico Labchip kit on the Agilent 2100 Bioanalyzer (Agilent Technologies). The Ovation PicoSL WTA System v2 kit (NuGEN) was used to amplify cDNA from 50ng total RNA. The MinElute Reaction Cleanup Kit (Qiagen) option was used to purify cDNA
|
| Label |
Cy3
|
| Label protocol |
1 μg was labelled using a one-color DNA labelling kit (NimbleGen, Roche)
|
| |
|
| Hybridization protocol |
Hybridization was performed according to NimbleGen Systems Inc.instructions following their standard operating protocol.
|
| Scan protocol |
Hybridised arrays were scanned at 2μm resolution with the Agilent High-resolution C Microarray Scanner (Agilent)
|
| Data processing |
Microarray images were processed using DEVA v1.2.1 software (Roche) to obtain a report containing the signal intensity values corresponding to each probe. The raw data was pre-processed using the DEVA v1.2.1 software by log2 transformation followed by RMA normalisation and summarisation to yield a signal intensity value for each probe set.
|
| |
|
| Submission date |
Jul 05, 2016 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Falko Steinbach |
| Organization name |
Univ of Surrey
|
| Street address |
Daphne Jackson Road
|
| City |
Guildford |
| ZIP/Postal code |
GU2 7AL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL22116 |
| Series (1) |
| GSE84031 |
Equine dendritic cells generated with horse serum have enhanced functionality in comparison to dendritic cells generated with fetal bovine serum |
|
