|
| Status |
Public on Jul 20, 2016 |
| Title |
NK.IL2.250U.8h#4_reanalyzed |
| Sample type |
RNA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
cytokine: IL2
treatment time: 8hrs
age: 6 Weeks Old
genetic background: C57BL/6
Sex: Male
cell family: NK cell
phenotype markers: NK1.1
source prep description: EasySep Neg Sel. & IL2 in vitro
separation method: Dissection / Trimming
amplification technique: Ambion WT Expression Kit
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZol
|
| Label |
Biotin
|
| Label protocol |
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
|
| |
|
| Hybridization protocol |
Ambion WT Expression Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner
|
| Data processing |
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| |
|
| Submission date |
Jul 19, 2016 |
| Last update date |
Jul 20, 2016 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE84564 |
NK cell response to IL2 in vitro |
| GSE84568 |
Immuno-genomic effects of JAK blockade |
|
| Relations |
| Reanalysis of |
GSM2241957 |
