|
| Status |
Public on Feb 23, 2017 |
| Title |
SW1116-Vector |
| Sample type |
SRA |
| |
|
| Source name |
control, SW1116 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: colorectal cancer line SW1116
genotype/variation: control
|
| Treatment protocol |
We used Ajuba OE and Vector virus to infect SW-1116 cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
DMEM medium were removed, PBS was washed three times, and RNA was harvested using Trizol reagent. RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
alignment: TopHat v2.0.14 using default settings
expression levels: Cufflinks v2.2.1, Cuffdiff and Cuffnorm using default settings
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text file includes RPKM values for each Sample
|
| |
|
| Submission date |
Jul 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jinsong Yan |
| E-mail(s) |
yanjsdmu@dmu.edu.cn
|
| Organization name |
Dalian Medical University
|
| Department |
Institute of Cancer Stem Cell
|
| Street address |
9 west section, Lvshun south road, Dalian, Liaoning Province, China
|
| City |
Dalian |
| ZIP/Postal code |
116044 |
| Country |
China |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE84902 |
The LIM protein AJUBA promotes colorectal cancer cell survival through suppression of JAK1/STAT1/IFIT2 network |
|
| Relations |
| BioSample |
SAMN05449204 |
| SRA |
SRX1980063 |
