|
| Status |
Public on Jun 20, 2008 |
| Title |
Placenta tissue from patients with severe preeclampsia, replicate 6 of 17 |
| Sample type |
RNA |
| |
|
| Source name |
total RNA from placenta tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Placenta tissue
|
| Growth protocol |
Patient material
|
| Extracted molecule |
total RNA |
| Extraction protocol |
MagNa Pure Compact RNA Isolation Kit (Roche Applied Science)
|
| Label |
Chemiluminescent Fluorescence (Digoxigenin)
|
| Label protocol |
0.5 micrograms of total RNA was processed into digoxigenen (DIG)- labeled cRNA using the Applied Biosystems NanoAmp™ RT-IVT Labeling kit (Applied Biosystems)
|
| |
|
| Hybridization protocol |
The labelled DIG-cRNA (10 micrograms per microarray) was then injected into each microarray hybridization chamber. Following hybridization at 55°C for 16 hours, the unbound material was washed from the microarrays. Features that retained bound DIG- labeled cRNA were visualized using the Applied Biosystems Chemiluminescent Detection Kit. Anti-GIG alkaline phosphatase (Roche Applied Science) was used to hydrolyze a chemiluminescence substrate to generate light at 458 nm.
|
| Scan protocol |
Images were quantified using the integrated ABI1700 software
|
| Description |
Biological replicate 32 of 43. Placenta tissue from patients with severe preeclampsia
|
| Data processing |
The data were pre-processed using the R software (version 2.4.1). A quantile normalization (ABarray package) was carried out prior to further analysis. This comes in addition to the internal preprocessing and filtering carried out by the ABI 1700 software (http=//www.amebioscience.com/ABI1700.htm).
|
| |
|
| Submission date |
Aug 29, 2007 |
| Last update date |
Nov 13, 2015 |
| Contact name |
Ruth Hracky Paulssen |
| E-mail(s) |
ruthp@fagmed.uit.no
|
| Phone |
+47 77645480
|
| Fax |
+47 77644650
|
| URL |
http://www.unn.no/labforum
|
| Organization name |
Universitetet i Tromsø
|
| Department |
Institute of Clinical Medicine
|
| Lab |
Molecular Medical Research
|
| Street address |
MH-Bygget Breivika
|
| City |
Tromsø |
| ZIP/Postal code |
N-9037 |
| Country |
Norway |
| |
|
| Platform ID |
GPL2986 |
| Series (1) |
| GSE10588 |
Placental gene expression in severe preeclampsia. |
|
| Relations |
| Reanalyzed by |
GSE75010 |
