|
| Status |
Public on Feb 14, 2017 |
| Title |
whole blood genomic DNA of sample SE1621 |
| Sample type |
genomic |
| |
|
| Source name |
smoker whole blood genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: smoker
tissue: whole blood
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Nucleated DNA from whole blood was collected after sucrose-induced osmotic lysis of cells followed by phenol-chloroform extraction and DNA was stored in Tris-EDTA buffer at -20°C.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
500 ng of DNA was bisulfite converted using the EZ-DNA Methylation kit (Zymo Research)
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Data processing |
ChAMP
The 'Matrix_processed.txt' contains Average Beta (normalized using ChAMP) values
|
| |
|
| Submission date |
Aug 04, 2016 |
| Last update date |
Feb 14, 2017 |
| Contact name |
Xuting Wang |
| E-mail(s) |
wang21@niehs.nih.gov
|
| Phone |
9842873840
|
| Organization name |
NIEHS/NIH
|
| Street address |
111 TW Alexander Dr
|
| City |
Research Triangle Park |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE85210 |
Differential methylation analysis in human whole blood DNA from healthy smokers and non-smokers |
|
