|
| Status |
Public on Nov 01, 2007 |
| Title |
H69 tumor post-PDT, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
NCI-H69 xenografts, PDT treated and control untreated
|
| Organism |
Homo sapiens |
| Characteristics |
NCI-H69 human small cell lung cancer
|
| Treatment protocol |
Sample tumor collected 4 hours after PDT treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed from flash frozed/pulverized samples according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 micrograms total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 micrograms of cRNA wer hybridized for 16 hr at 45C on GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCOS Scanner 3000.
|
| Description |
Early gene expression data from xenograft tumors following sensitizing and PDT treatment.
|
| Data processing |
Data was analyzed with Agilent Genespring GX v. 7.3.1 using Affymetrix GCOS generated flag data from scaled CHP files, and RMA-normalized CEL files.
|
| |
|
| Submission date |
Aug 30, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Kate McInnerney |
| E-mail(s) |
kmcinnerney@montana.edu
|
| Phone |
406-994-5666
|
| Fax |
406-994-4926
|
| URL |
http://cores.montana.edu
|
| Organization name |
Montana State University
|
| Department |
Microbiology
|
| Lab |
Functional Genomics Facility
|
| Street address |
109 Lewis Hall
|
| City |
Bozeman |
| State/province |
MT |
| ZIP/Postal code |
59717 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE8920 |
Early expression data of H69 xenograft tumors following PDT treatment. |
|
