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Status |
Public on Feb 08, 2020 |
Title |
Pancreactic islets from 12-week old hIAPP-Tg mouse 6 |
Sample type |
RNA |
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Source name |
Pancreatic islets
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Organism |
Mus musculus |
Characteristics |
strain: FVB/N-Tg(Ins2-IAPP)RFHSoel/J tissue: Islets of Langerhans genotype: hIAPP Tg age: 12 weeks
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Treatment protocol |
Wild type and hIAPP-Tg mice were fed ad libitum with standard chow diet for 12 weeks.
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Growth protocol |
Islets from wild type and hIAPP-Tg mice were isolated at week 12.
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol (R) reagent according to manufacturer protocol.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (150 ng starting total RNA).
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Hybridization protocol |
Following fragmentation, cRNA was hybridized to Genechip Mouse 430 2.0 Affymetrix arrays, washed and stained according to the standard Affymetrix protocol.
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Scan protocol |
GeneChips were scanned according to the standard Affymetrix protocol (Affymetrix Genechip Command Console program 3.0.0.1214, scanner GCS 3000-7G)
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Description |
Global gene expression of pancreactic islets from 12-week old hIAPP-Tg mouse
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Data processing |
Expression data were normalized with Robust Multi-array Average (RMA) from the affy package (www.bioconductor.org)
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Submission date |
Aug 09, 2016 |
Last update date |
Feb 08, 2020 |
Contact name |
Gema Alcarraz-Vizán |
E-mail(s) |
alcarraz@clinic.ub.es
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Organization name |
IDIBAPS
|
Department |
Diabetes & Obesity Laboratory
|
Street address |
Rossello 149
|
City |
Barcelona |
ZIP/Postal code |
08036 |
Country |
Spain |
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Platform ID |
GPL1261 |
Series (1) |
GSE85380 |
Global gene expression profile of hIAPP-Tg mice |
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