|
| Status |
Public on Feb 08, 2020 |
| Title |
Pancreactic islets from 12-week old hIAPP-Tg mouse 6 |
| Sample type |
RNA |
| |
|
| Source name |
Pancreatic islets
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB/N-Tg(Ins2-IAPP)RFHSoel/J
tissue: Islets of Langerhans
genotype: hIAPP Tg
age: 12 weeks
|
| Treatment protocol |
Wild type and hIAPP-Tg mice were fed ad libitum with standard chow diet for 12 weeks.
|
| Growth protocol |
Islets from wild type and hIAPP-Tg mice were isolated at week 12.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol (R) reagent according to manufacturer protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (150 ng starting total RNA).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized to Genechip Mouse 430 2.0 Affymetrix arrays, washed and stained according to the standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned according to the standard Affymetrix protocol (Affymetrix Genechip Command Console program 3.0.0.1214, scanner GCS 3000-7G)
|
| Description |
Global gene expression of pancreactic islets from 12-week old hIAPP-Tg mouse
|
| Data processing |
Expression data were normalized with Robust Multi-array Average (RMA) from the affy package (www.bioconductor.org)
|
| |
|
| Submission date |
Aug 09, 2016 |
| Last update date |
Feb 08, 2020 |
| Contact name |
Gema Alcarraz-Vizán |
| E-mail(s) |
alcarraz@clinic.ub.es
|
| Organization name |
IDIBAPS
|
| Department |
Diabetes & Obesity Laboratory
|
| Street address |
Rossello 149
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE85380 |
Global gene expression profile of hIAPP-Tg mice |
|
