|
| Status |
Public on Nov 22, 2017 |
| Title |
F33: Monocytes DNA from healthy female |
| Sample type |
genomic |
| |
|
| Source name |
monocytes
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
cell type: CD14
age (years): 33
clinical phenotype: Healthy
cell type: CD14 purified from PBMC
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
PBMCs were purified from 60 ml blood by Ficoll density gradient centrifugation (U16 NovaMed). The CD14 subset was isolated using EasySep™ Human Monocyte Enrichment Kit (#19059 Stemcell). Recovery was 65-80% with a purity > 95% for all cell-subsets. From each sample both RNA and DNA was extracted using AllPrep kit (#80204 QIAGENE) according to manufacturer's protocol. DNA concentration was measured by picogreen and stored at 4°C until use. RNA quantity and quality was measured by NanoDrop and Agilent BioAnalyzer respectively and stored at -80°C until use.
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Satndart Illumina label protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
CD14 isolated using EasySep™#19059 Stemcell
Singleton
Array Run I
F33
|
| Data processing |
The methylation β-values were extracted from iDAT files and normalized using ChAMP R pipeline, .
|
| |
|
| Submission date |
Aug 15, 2016 |
| Last update date |
Nov 22, 2017 |
| Contact name |
Sonia Berrih-Aknin |
| E-mail(s) |
sonia.berrih-aknin@upmc.fr
|
| Organization name |
UPMC - Inserm UMRS 974, CNRS FRE3617
|
| Department |
Sorbonne Universités
|
| Lab |
Center of Research in Myology
|
| Street address |
105 Boulevard de l’Hôpital
|
| City |
Paris |
| State/province |
State... |
| ZIP/Postal code |
75651 |
| Country |
France |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE85647 |
Methylation and expression profiles of monocytes in monozygotic twins: role in Myasthenia Gravis [methylation profiling] |
| GSE85649 |
Methylation and expression profiles of monocytes in monozygotic twins: role in Myasthenia Gravis |
|
