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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 17, 2016 |
| Title |
MDS_314 |
| Sample type |
SRA |
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| Source name |
Bone Marrow
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| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone Marrow
mutation: p.K700E
cell population: Total blood
library prep: TrueSeq
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted using the PrepEase RNA Spin Kit
Human RNA libraries were prepared for sequencing using TrueSeq, while mouse RNA libraries were prepared using the NEBNext Ultra Library Preparation Kit
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
Raw data were aligned to hg19 using the STAR alignment package
Unique junction counts as determined by STAR are output to a tabular file
Genome_build: hg19 for human samples, mm10 for mouse
Supplementary_files_format_and_content: Raw junction counts for each sample as output by STAR
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| Submission date |
Aug 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Silvia Buonamici |
| Organization name |
H3 Biomedicine
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| Street address |
300 Technology Square #5
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platform ID |
GPL11154 |
| Series (1) |
| GSE85712 |
Physiologic expression of Sf3b1K700E causes impaired erythropoieses, aberrant splicing, and sensitivity to pharmacologic spliceosome modulation |
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| Relations |
| Reanalyzed by |
GSE122515 |
| BioSample |
SAMN05580179 |
| SRA |
SRX2027198 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM2282083_GS000576.txt.gz |
9.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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