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Sample GSM22862 Query DataSets for GSM22862
Status Public on May 21, 2004
Title B chip Version2: time 15 day Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 15 day Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 198.676 191.174 104.443 86.2752 137 135.5 35 33.5
2 NULL 0 207.657 170.699 107.102 99.9398 144 113.5 45 36.5
3 -4.11E-02 0 1150.04 214.38 956.623 141.937 1040 161 811 93
4 1.11E-01 0 267.869 190.975 171.517 112.34 216 127 97.5 64
5 3.92E-01 0 1969.54 228.586 1241.9 145.271 1781 215 1208 86.5
6 -7.17E-02 0 510.769 207.397 358.652 135.801 416 170 284 68
7 3.84E-01 0 9518.98 291.87 5104.84 187.559 9701 245 5121 130.5
8 4.87E-01 0 305.293 212.775 149.625 125.211 232 139.5 94 51.5
9 -4.11E-01 0 356.852 223.197 223.703 120.609 287 192 147 49
10 NULL 0 214.027 182.4 96.1832 109.35 150.5 137 31 53
11 NULL 0 209.14 177.626 101.588 105.418 158 120.5 32 32.5
12 NULL 0 223.424 197.8 110.733 105.846 153 135 43 47.5
13 4.66E-01 0 576.9 227.426 287.454 104.993 489 161.5 203 39.5
14 NULL 0 226.873 230.867 105.482 84.7934 158 169 47 26
15 6.05E-02 0 569.991 232.414 363.149 121.21 477 183 275 61.5
16 -4.64E-01 0 287.818 223.933 173.523 126.133 217 180 105 51.5
17 6.42E-02 0 774.393 295.302 512.053 147.973 661 212 473 76
18 2.81E-01 0 571.754 237.019 324.916 121.415 484 187.5 240 65.5
19 2.55E-01 0 254.788 185.697 132.378 102.713 205 124.5 61.5 40
20 NULL 0 217.486 193.981 124.37 104.826 148 98 58 40

Total number of rows: 14720

Table truncated, full table size 934 Kbytes.




Supplementary data files not provided

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