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Status
Public on May 21, 2004
Title
B chip Version2: time 15 day Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 15 day Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
198.676
191.174
104.443
86.2752
137
135.5
35
33.5
2
NULL
0
207.657
170.699
107.102
99.9398
144
113.5
45
36.5
3
-4.11E-02
0
1150.04
214.38
956.623
141.937
1040
161
811
93
4
1.11E-01
0
267.869
190.975
171.517
112.34
216
127
97.5
64
5
3.92E-01
0
1969.54
228.586
1241.9
145.271
1781
215
1208
86.5
6
-7.17E-02
0
510.769
207.397
358.652
135.801
416
170
284
68
7
3.84E-01
0
9518.98
291.87
5104.84
187.559
9701
245
5121
130.5
8
4.87E-01
0
305.293
212.775
149.625
125.211
232
139.5
94
51.5
9
-4.11E-01
0
356.852
223.197
223.703
120.609
287
192
147
49
10
NULL
0
214.027
182.4
96.1832
109.35
150.5
137
31
53
11
NULL
0
209.14
177.626
101.588
105.418
158
120.5
32
32.5
12
NULL
0
223.424
197.8
110.733
105.846
153
135
43
47.5
13
4.66E-01
0
576.9
227.426
287.454
104.993
489
161.5
203
39.5
14
NULL
0
226.873
230.867
105.482
84.7934
158
169
47
26
15
6.05E-02
0
569.991
232.414
363.149
121.21
477
183
275
61.5
16
-4.64E-01
0
287.818
223.933
173.523
126.133
217
180
105
51.5
17
6.42E-02
0
774.393
295.302
512.053
147.973
661
212
473
76
18
2.81E-01
0
571.754
237.019
324.916
121.415
484
187.5
240
65.5
19
2.55E-01
0
254.788
185.697
132.378
102.713
205
124.5
61.5
40
20
NULL
0
217.486
193.981
124.37
104.826
148
98
58
40
Total number of rows: 14720 Table truncated, full table size 934 Kbytes .Supplementary data files not provided