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Sample GSM228810 Query DataSets for GSM228810
Status Public on Sep 12, 2007
Title HEL 48h 1
Sample type RNA
Source name Rag2-GFP, HEL-Ig BM cells
Organism Mus musculus
Characteristics Strain C57BL/9
Treatment protocol HEL treated samples were washed after 5 days, replaced with new media with no IL-7, but 1ug/mL HEL. Control samples were treated the same except no HEL in the media. HEL Mlo samples were sorted for IgMa low, IgDneg. HEL 48h samples were sorted for B220+ IgMahi, GFP+ cells after 48h in HEL. BM 48h Ctrl samples were sorted for B220+ IgMahi GFPneg after 48h in culture with no HEL.
Growth protocol 5 day culture in RPMI + 10% FBS, Pen/Strep, L-glut, 2-ME, 16ng/mL IL-7
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy protocol
Label biotin
Label protocol Ambion cDNA synthesis with T7 oligo dT primer (Array Script). Labeling with biotin-11-CTP and biotin-16-UTP.
Hybridization protocol Standard Affymetrix procedures
Scan protocol Standard Affymetrix procedures
Description LET_1103114
Data processing MAS5.0
Submission date Sep 11, 2007
Last update date Aug 14, 2011
Contact name Lina Tze
Phone 011-61-2-6125-2031
Organization name John Curtin School of Medical Research
Street address
City Canberra
State/province ACT
ZIP/Postal code 2601
Country Australia
Platform ID GPL32
Series (1)
GSE2227 The role of basal immunoglobulin signaling in immature B cell development

Data table header descriptions
VALUE MAS 5.0 signal

Data table
100000_at 33.24
100001_at 47.42
100002_at 62.82
100003_at 313.48
100004_at 1373.25
100005_at 1955.33
100006_at 73.61
100007_at 2947.19
100008_at 502.92
100009_r_at 8.54
100010_at 1278.76
100011_at 3059.11
100012_at 20719.46
100013_at 1697.42
100014_at 1704.11
100015_at 104.07
100016_at 603.06
100017_at 457.98
100018_at 1037.7
100019_at 88.4

Total number of rows: 12654

Table truncated, full table size 209 Kbytes.

Supplementary file Size Download File type/resource
GSM228810.CEL.gz 2.7 Mb (ftp)(http) CEL
GSM228810.CHP.gz 4.6 Mb (ftp)(http) CHP
GSM228810.EXP.gz 469 b (ftp)(http) EXP
Processed data included within Sample table
Processed data provided as supplementary file

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