|
Status |
Public on Nov 08, 2016 |
Title |
Post-partum 2 weeks ID12_set2 |
Sample type |
RNA |
|
|
Source name |
PBMC
|
Organism |
Homo sapiens |
Characteristics |
source: human condition: Post-partum 2 weeks
|
Treatment protocol |
NA
|
Growth protocol |
NA
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the Rneasy RNA Isolation Kit according to manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cDNAs were prepared using Nugen Ovation WTA protocol from 25 ng total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, 5 micrograms of cDNA were diluted into 220 microlitres of hybridization cocktail, with 200 microlitres hybridized for 18 hr at 45 C on Human U133 Plus 2 Array in Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Data processing |
Data Processiing performed with TIBCO Spotfire and OmicsOffice
|
|
|
Submission date |
Aug 23, 2016 |
Last update date |
Nov 08, 2016 |
Contact name |
Gregory K Tharp |
E-mail(s) |
gktharp@emory.edu
|
Phone |
404-727-7797
|
Organization name |
Yerkes National Primate Research Center
|
Department |
Developmental and Cognitive Neuroscience
|
Lab |
Genomics Core
|
Street address |
954 Gatewood Dr
|
City |
Atlanta |
State/province |
GA |
ZIP/Postal code |
30329-4208 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE85960 |
Prolonged activation of innate antiviral gene signature after childbirth is determined by IFNL3 genotype |
|