|
| Status |
Public on Nov 08, 2016 |
| Title |
Post-partum 2 weeks ID12_set2 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
source: human
condition: Post-partum 2 weeks
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Rneasy RNA Isolation Kit according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNAs were prepared using Nugen Ovation WTA protocol from 25 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 micrograms of cDNA were diluted into 220 microlitres of hybridization cocktail, with 200 microlitres hybridized for 18 hr at 45 C on Human U133 Plus 2 Array in Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
Data Processiing performed with TIBCO Spotfire and OmicsOffice
|
| |
|
| Submission date |
Aug 23, 2016 |
| Last update date |
Nov 08, 2016 |
| Contact name |
Gregory K Tharp |
| E-mail(s) |
gktharp@emory.edu
|
| Phone |
404-727-7797
|
| Organization name |
Yerkes National Primate Research Center
|
| Department |
Developmental and Cognitive Neuroscience
|
| Lab |
Genomics Core
|
| Street address |
954 Gatewood Dr
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30329-4208 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE85960 |
Prolonged activation of innate antiviral gene signature after childbirth is determined by IFNL3 genotype |
|
