|
| Status |
Public on Aug 25, 2016 |
| Title |
RNA from mouse liver 135 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from mouse liver 135
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
macronutrient in p/c/f: Gene expression data from mouse 135 High energy 5/48/48
|
| Treatment protocol |
Mice were placed on one of 25 different diets from 3 weeks of age and fed ad libitum. Mice were left to age until 15 months old and culled for analyses of tissues
|
| Growth protocol |
Mice were placed on one of 25 different diets from 3 weeks of age and fed ad libitum. Mice were maintained 3 per cage at 24-26C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed according to the manufacturer's instructions using the Qiagen AllPrep DNA/RNA/Protein Mini Kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on the Affymetrix Mouse Gene ST 2.1 Array plate. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with Bioconductor package for R
|
| |
|
| Submission date |
Aug 24, 2016 |
| Last update date |
Aug 25, 2016 |
| Contact name |
Samantha Solon-Biet |
| E-mail(s) |
samantha.biet@sydney.edu.au
|
| Phone |
61 2 8627 0430
|
| Organization name |
University of Sydney
|
| Department |
Charles Perkins Centre
|
| Street address |
The University of Sydney
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2043 |
| Country |
Australia |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE85998 |
Expression data from mouse liver |
|
