|
| Status |
Public on Feb 09, 2017 |
| Title |
195minLPS+c10 Rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Bone Marrow Derived Macrophages
|
| Organism |
Mus musculus |
| Characteristics |
genotype: IL10-/-RAG2-/-
strain: 129S6/SvEvTac
cell type: Bone Marrow Derived Macrophage
|
| Treatment protocol |
Untreated, LPS (1ng/mL) for 195min, LPS and IL-10 (1ng/mL) for 195min (c10), LPS for 195min and IL-10 for the last 30min of the stimulation (a10)
|
| Growth protocol |
Bone marrow precursors were grown in M-CSF conditioned media containing FBS for 5 days prior to stimulation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were rinsed in PBS and lysed in TRIzol, RNA was purified with qiagen RNeasy Columns
NEB next ribomal depletion followed by NEB Directional RNA Library Prep Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
195min LPS + IL-10 (c10)
|
| Data processing |
Trim_galore
Rbowtie
QuasR qCount
DESeq
Genome_build: mm10
Supplementary_files_format_and_content: csv of normalized expression values
|
| |
|
| Submission date |
Aug 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Bruce Horwitz |
| E-mail(s) |
bhorwitz@rics.bwh.harvard.edu
|
| Organization name |
Brigham and Women's Hospital
|
| Department |
Pathology
|
| Street address |
77 Ave Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE86167 |
Inhibition of inflammatory gene transcription by IL-10 is associated with rapid suppression of LPS-induced enhancer activation [RNA-Seq] |
| GSE86170 |
Inhibition of inflammatory gene transcription by IL-10 is associated with rapid suppression of LPS-induced enhancer activation |
|
| Relations |
| BioSample |
SAMN05713625 |
| SRA |
SRX2060946 |
