|
| Status |
Public on Dec 13, 2016 |
| Title |
miDicE16.5 KO Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Dicer cKO FP (miRNA)
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL6
developmental stage: E16.5
cell type: Dicer cKO
tissue: footpad skin
individual identifier: 257015510138A1_1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
E16.5 and E17.5 mouse embryos from timed-mating were euthanized and footpad skin was dissected out under a dissection microscope, and was frozen on dry ice immediately. Samples were kept in -80C reezer until use. The frozen footpads were pooled (3-5 embryos for one replicate), and miRNA-enriched total RNAs were isolated with mirVana miRNA isolation kit (Ambion, AM1560) according to manufacturer’s standard protocol. Isolated RNAs were kept in -80C freezer until use.
|
| Label |
Cy3
|
| Label protocol |
Total RNA was labeled using the Agilent miRNA Complete Labeling and Hyb Kit according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Total RNA was hybridized using the Agilent miRNA Complete Labeling and Hyb Kit according to the manufacturer's instructions.
|
| Scan protocol |
Slides were scanned with an Agilent DNA Microarray Scanner C by using AgilentG3_miRNA protocol, with a scan resolution of 3microns.
|
| Description |
miDicE16_KO_rep2
|
| Data processing |
Data are extracted with Agilent Feature Extraction Software.
The arrays are normalized using quantile method
The data were further processed with NIA ExAtlas utilities. See http://lgsun.grc.nia.nih.gov/exatlas for details.
|
| |
|
| Submission date |
Aug 31, 2016 |
| Last update date |
Dec 14, 2016 |
| Contact name |
Minoru S.H. Ko |
| E-mail(s) |
kom@mail.nih.gov
|
| Phone |
410-558-8359
|
| Organization name |
NIH
|
| Department |
National Institute on Aging
|
| Lab |
Lab of Genetics
|
| Street address |
251 Bayview Blvd, Suite 100, 10C
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
|
| Platform ID |
GPL22383 |
| Series (2) |
| GSE86295 |
miRNAs are required for post-induction stage sweat gland development [miRNA] |
| GSE86296 |
miRNAs are required for post-induction stage sweat gland development |
|
