|
| Status |
Public on Jan 01, 2008 |
| Title |
Mesenchymal clone 15.5 |
| Sample type |
mixed |
| |
|
| Channel 1 |
| Source name |
Genomic DNA extracted from H1 Human Embryonic Stem Cells
|
| Organism |
Homo sapiens |
| Characteristics |
Genomic DNA extracted from H1 ES cells
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Phenol/Chloroform Extraction
|
| Label |
Cy3
|
| Label protocol |
Sonicated genomic DNA was primed with random octamers and labeled using Klenow in the present of 5-(3-aminoallyl)-dUTP
|
| |
|
| Channel 2 |
| Source name |
Mesenchymal clone 15.5
|
| Organism |
Homo sapiens |
| Characteristics |
Mesenchymal clone 15.5 derived from human Oct4 knock-in ES cell lines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen on column kit
|
| Label |
Cy5
|
| Label protocol |
Total RNA was enriched for the polyA fraction using Oligotex mRNA Mini Kit (Qiagen). Enriched mRNA (250 ng) was primed using random hexamers and reversed transcribed using Superscript III (Invitrogen) in the presence of 5-(3-aminoallyl)-dUTP (Ambion).
|
| |
|
| |
| Hybridization protocol |
Hybridizations were performed in 3.6X SSC buffer with 35% formamide and 0.07% SDS at 42% C overnight.
|
| Scan protocol |
Arrays were washed, dried and scanned with the GenePix 4000B Scanner
|
| Description |
Total RNA from iPS clone was extracted using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's recommendations.
|
| Data processing |
Gene Expression data was extracted using NimbleScan Software v2.3
|
| |
|
| Submission date |
Sep 18, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Victor Ruotti |
| E-mail(s) |
ruotti@wisc.edu
|
| Phone |
608-890-0182
|
| Fax |
608-890-0167
|
| Organization name |
UW Madison
|
| Department |
Biotechnology
|
| Lab |
Thomson's Lab
|
| Street address |
425 Henry Mall
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL5876 |
| Series (1) |
| GSE9071 |
Reprogramming human somatic cells to a pluripotent state |
|
