|
| Status |
Public on Sep 01, 2017 |
| Title |
carbon source glucose 3 |
| Sample type |
RNA |
| |
|
| Source name |
carbon source glucose
|
| Organism |
Corynebacterium glutamicum ATCC 13032 |
| Characteristics |
carbon source: glucose
|
| Treatment protocol |
Samples were collected during the mid-logarithmic growth phase in minimal medium with added glucose (control sample: 100 mM) or ferulic acid as the sole carbon source (3 mM), respectively.
|
| Growth protocol |
C. glutamicum strains were routinely grown in LB medium or in mineral salts medium supplemented with 0.05 g l-1 of yeast extract to meet the requirement of vitamins for the strains on a rotary shaker at 30°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
RNA was labled by low Input Quick Amp Labeling Kit, One-Color, following the manufacture' instructions.
|
| |
|
| Hybridization protocol |
Each Slide was hybridized with 600 ng Cy3-labeled cRNA using Gene Expression Hybridization Kit in Hybridization Oven according to the manufacturer's insrtuctions.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2565CA) using one color scan, Scan resolution 3um, Dye channel is set to Green).
|
| Description |
control group 3
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7 (Agilent) to obtain background subtracted and spatially detrended Processed Signal intensities.
|
| |
|
| Submission date |
Sep 07, 2016 |
| Last update date |
Sep 01, 2017 |
| Contact name |
Can Chen |
| E-mail(s) |
cc7309@163.com
|
| Organization name |
Northwest A&F University
|
| Street address |
No.3 Taicheng Road
|
| City |
Yangling |
| ZIP/Postal code |
712100 |
| Country |
China |
| |
|
| Platform ID |
GPL22357 |
| Series (1) |
| GSE86537 |
Global transcriptomic analysis of the response of Corynebacterium glutamicum to ferulic acid |
|
