|
| Status |
Public on Sep 14, 2017 |
| Title |
YoungAdult_24hr_Chuong508 |
| Sample type |
SRA |
| |
|
| Source name |
YoungAdult, 24 hrs
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/C
developmental stage: Young adult
age: about 2 months
cell type: cultured dorsal skin dissociated cells
culture time: 24 hrs
|
| Treatment protocol |
Reconstitution and in vitro culture
|
| Growth protocol |
37℃ with 5% CO2 in incubator
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA of dissociated culture cells were extracted using Trizol reagent
Illumina TruSeq RNA Sample Preparation V2
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
The RNA-Seq alignment was performed by STAR 2.4.1d. Mouse mm9 downloaded from the UCSC Genome Browser.
The quantification was performed by htseq-count 0.6.0 using the union mode
Genome_build: mm9
Supplementary_files_format_and_content: txt files including raw counts of sequencing reads for refGene produced by htseq-count
|
| |
|
| Submission date |
Sep 15, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Mingxing Lei |
| E-mail(s) |
lmxgoodluck@163.com
|
| Phone |
3234422845
|
| Organization name |
University of Southern California
|
| Street address |
2011 Zonal Ave HMR 304A
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE86955 |
Newborn and young adult (2 months) mouse dorsal skin cell culture time-series RNA-Seq |
|
| Relations |
| BioSample |
SAMN05773338 |
| SRA |
SRX2164782 |
