|
| Status |
Public on Oct 23, 2017 |
| Title |
ED13_2 |
| Sample type |
SRA |
| |
|
| Source name |
B. germanica embryo day 13
|
| Organism |
Blattella germanica |
| Characteristics |
developmental stage: embryo day 13
tissue: Whole body
|
| Treatment protocol |
We sequenced 22 small RNA-seq libraries, two replicates for each one of the 11 developmental stages selected according to Juvenil hormone and Ecdysone hormone patterns
|
| Growth protocol |
Blattella germanica specimens used for small RNA-seq analysis were obtained from a population reared at the Institut de Biologia Evolutiva (CSIC-UPF). Conditions were 70% humidity at 30 ºC and a commercial dog food diet.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Mini Kit (Qiagen)
NEBNext® Small RNA Library Prep Set for Illumina®
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
miRNA_table_genome.csv
|
| Data processing |
Quality control with FastQC
Read filtering and trimming with Trimmomatic
Reads mapping with Bowtie
miRNA counts with FeatureCounts
Genome_build: https://www.hgsc.bcm.edu/arthropods/german-cockroach-genome-project
|
| |
|
| Submission date |
Sep 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Guillem Ylla |
| E-mail(s) |
guillem.ylla@ibe.upf-csic.es
|
| Organization name |
Institute of Evolutionary Biology
|
| Lab |
Belles & Piulachs Labs
|
| Street address |
Passeig Marítim de la Barceloneta, 37
|
| City |
Barcelona |
| State/province |
Catalonia |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL22038 |
| Series (1) |
| GSE87031 |
Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types |
|
| Relations |
| BioSample |
SAMN05785005 |
| SRA |
SRX2172578 |
