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Sample GSM2331271 Query DataSets for GSM2331271
Status Public on Feb 01, 2017
Title normal control, biological rep 6
Sample type RNA
 
Source name Whole retina from normoglycemic animals @12 weeks
Organism Mus musculus
Characteristics background strain: C57Bl6/J
tissue: Whole retina
disease: normoglycemic
beta cells transplantation: no
streptozotocin: Ctrl
Treatment protocol Enucleated Eyes were snapp frozen in liquid nitrogen and stored at -80°C till further preperation. Extraction of the retina was performed at 0°C and immediately suspended in Trizol.
Growth protocol Diabetes was induced by intra peritoneal injection of streptozotocin (160 mg/kg/bodyweight; Sigma) in 8-week-old outbred male C57BL6/J mice weighing 24-26 g. After confirmation of stable hyperglycemia (blood glucose >15 mM 7 d after injection), hyperglycemic mice were randomly assigned to receive either standard chow or chow containing benfotiamine (80 mg per kg body weight per day). Non-diabetic animals served as controls. Body weight and blood glucose values were monitored at regular intervals. Animals were housed with a 12-/12-h light/dark cycle with ad libitum access to standard or benfotiamine containing rodent chow. Termination occured at 6 weeks and 12 weeks after diabetes induction.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description Gene expression data from normal control C57Bl6/J mice after 12 weeks
Data processing The data were analyzed with a commercial software called JMP Genomics, version 7, from SAS. Gene expression profiling was performed using arrays of human Mogene-2_0-type from Affymetrix. A Custom CDF Version 19 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
 
Submission date Sep 28, 2016
Last update date Mar 12, 2018
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL20710
Series (1)
GSE87433 Hyperglycemic Memory – New insights into a thought to be known topic

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
100009600 5.336914063
100009609 5.149414063
100009614 5.2421875
100009664 5.299804688
100012 5.24609375
100017 5.782226563
100019 8.251953125
100033459 5.09375
100034251 5.619140625
100034675 5.288085938
100034729 5.262695313
100034739 5.428710938
100034748 5.624023438
100036518 5.209960938
100036520 5.47265625
100036521 5.958984375
100036523 6.638671875
100036537 6.571289063
100036569 5.161132813
100036768 6.568359375

Total number of rows: 24973

Table truncated, full table size 443 Kbytes.




Supplementary file Size Download File type/resource
GSM2331271_Friedrichs_110314_1095_NC_3M_MoGene-2_0-st.CEL.gz 9.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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