|
| Status |
Public on Feb 01, 2017 |
| Title |
diabetic control, biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Whole retina from diabetic animals @12 weeks
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57Bl6/J
tissue: Whole retina
disease: diabetic
beta cells transplantation: no
streptozotocin: Yes
|
| Treatment protocol |
Enucleated Eyes were snapp frozen in liquid nitrogen and stored at -80°C till further preperation. Extraction of the retina was performed at 0°C and immediately suspended in Trizol.
|
| Growth protocol |
Diabetes was induced by intra peritoneal injection of streptozotocin (160 mg/kg/bodyweight; Sigma) in 8-week-old outbred male C57BL6/J mice weighing 24-26 g. After confirmation of stable hyperglycemia (blood glucose >15 mM 7 d after injection), hyperglycemic mice were randomly assigned to receive either standard chow or chow containing benfotiamine (80 mg per kg body weight per day). Non-diabetic animals served as controls. Body weight and blood glucose values were monitored at regular intervals. Animals were housed with a 12-/12-h light/dark cycle with ad libitum access to standard or benfotiamine containing rodent chow. Termination occured at 6 weeks and 12 weeks after diabetes induction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression data from adult C57Bl6/J mice 12 weeks after STZ diabetes induction
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 7, from SAS. Gene expression profiling was performed using arrays of human Mogene-2_0-type from Affymetrix. A Custom CDF Version 19 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Sep 28, 2016 |
| Last update date |
Mar 12, 2018 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL20710 |
| Series (1) |
| GSE87433 |
Hyperglycemic Memory – New insights into a thought to be known topic |
|
