|
| Status |
Public on Apr 12, 2017 |
| Title |
sham_12hr_animal18 |
| Sample type |
RNA |
| |
|
| Source name |
liver from animals with sham surgery, 12hours after surgery
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Liver
surgery: sham
time following surgery: 12hr
gender: male
strain: Sprague-Dawley CD/IGS
animal number: 18
excluded animal: No
|
| Treatment protocol |
We conducted a rat bile duct ligation (BDL) study, a common model for cholestatic liver injury and BDH, and monitored injury and expression changes from 3 hours through 14 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were homogenized in Qiagen RLT buffer, and RNA isolated using Qiagen RNeasy columns per manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
RNA was then labeled and hybridized to RG230-2 microarrays (Affymetrix) according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Affymetrix hyb protocol: EukGE-WS2v5_450
|
| Scan protocol |
.CEL files were generated using Affymetrix GeneChip Command Console
|
| Data processing |
MAS5 signal intensities were generated using Bioconductor's Affy package. Data was scaled to a target of 1500.
|
| |
|
| Submission date |
Oct 06, 2016 |
| Last update date |
Apr 13, 2017 |
| Contact name |
Keith M Goldstein |
| E-mail(s) |
goldsteinkm@lilly.com
|
| Organization name |
Eli Lilly & Co.
|
| Department |
Investigative Toxicology
|
| Lab |
Keith Goldstein
|
| Street address |
Lilly Corporate Center
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46285 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE87696 |
Toxicogenomic module associations with pathogenesis: A network based approach to understanding drug toxicity |
|
