|
Status |
Public on May 05, 2017 |
Title |
aHetgWT 1 |
Sample type |
SRA |
|
|
Source name |
Heart
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 genotype: ERRa Het/ERRg WT tissue: Heart age: post natal day 16
|
Extracted molecule |
total RNA |
Extraction protocol |
Hearts were quickly dissected and flash frozen in liquid nitrogen. RNA was harvested using RNAzol reagent. RNA libraries were prepared at BGI@CHOP using Illumina TruSeq RNA Sample Prep Kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
ERRa Het/ERRg WT
|
Data processing |
After QC, clean reads were aligned with reference genome using SOAP2 Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated Genome_build: mm10 Supplementary_files_format_and_content: excel file containing gene ID, rpkm and other relevant information
|
|
|
Submission date |
Oct 14, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Liming Pei |
E-mail(s) |
peil@email.chop.edu
|
Phone |
267-425-2118
|
Organization name |
Children's Hospital of Philadelphia/University of Pennsylvania
|
Department |
Pathology and Laboratory Medicine
|
Street address |
3501 Civic Center Blvd. CTRB 6018
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19104 |
Country |
USA |
|
|
Platform ID |
GPL13112 |
Series (1) |
GSE88761 |
ERRa/ERRg KO heart gene expression analysis |
|
Relations |
BioSample |
SAMN05908538 |
SRA |
SRX2245590 |