|
| Status |
Public on May 05, 2017 |
| Title |
aHetgKO 1 |
| Sample type |
SRA |
| |
|
| Source name |
Heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: ERRa Het/ERRg KO
tissue: Heart
age: post natal day 16
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hearts were quickly dissected and flash frozen in liquid nitrogen. RNA was harvested using RNAzol reagent.
RNA libraries were prepared at BGI@CHOP using Illumina TruSeq RNA Sample Prep Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
ERRa Het/ERRg KO
|
| Data processing |
After QC, clean reads were aligned with reference genome using SOAP2
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated
Genome_build: mm10
Supplementary_files_format_and_content: excel file containing gene ID, rpkm and other relevant information
|
| |
|
| Submission date |
Oct 14, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Liming Pei |
| E-mail(s) |
peil@email.chop.edu
|
| Phone |
267-425-2118
|
| Organization name |
Children's Hospital of Philadelphia/University of Pennsylvania
|
| Department |
Pathology and Laboratory Medicine
|
| Street address |
3501 Civic Center Blvd. CTRB 6018
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE88761 |
ERRa/ERRg KO heart gene expression analysis |
|
| Relations |
| BioSample |
SAMN05908557 |
| SRA |
SRX2245592 |
