|
| Status |
Public on Oct 09, 2007 |
| Title |
pcDNA3.1 r4 |
| Sample type |
RNA |
| |
|
| Source name |
293 cells with pcDNA3.1/V5/HisA
|
| Organism |
Homo sapiens |
| Characteristics |
human embryonic kidney cells expressing control vector pcDNA3.1/V5/HisA
|
| Treatment protocol |
293 cells were stably expressing either miR-155, mir-K12-11, or pcDNA vector control while selected under 100 ug/mL G418
|
| Growth protocol |
grow 293 cells to 80% confluence in DMEM with 10% FBS, collected 2 samples each 2 days apart for a total of 8 (4 each miR-155 or miR-K12-11) experimental and 4 control samples (pCDNA3.1)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG133 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix F450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
|
| Description |
gene expression from 293 cells with pmiRNA cluster
|
| Data processing |
The data were normalized and modelled with DNA-Chip Analyzer (dChip) Version 1.3 (2/23/05)
|
| |
|
| Submission date |
Oct 09, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
M Cecilia Lopez |
| E-mail(s) |
mclopez@ufl.edu
|
| Phone |
352 273-8133
|
| Organization name |
University of Florida
|
| Department |
Molecular Genetics and Microbiology
|
| Lab |
Microarray lab 103
|
| Street address |
1376 Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610-3610 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9264 |
Kaposi's Sarcoma-associated Herpesvirus Encodes an Ortholog of miR-155 |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
