|
| Status |
Public on Oct 17, 2017 |
| Title |
Surgical5 |
| Sample type |
RNA |
| |
|
| Source name |
Surgical: Human Islets LCM
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: pancreas
cell type: Islets LCM
age: 81
gender: M
bmi: 23
|
| Growth protocol |
LCM samples were obtained from selected beta cell rich autofluorescent areas of islets or from generalized non-selected areas LCM of islet tissue.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PicoPure, Dnase treatment, RiboAmp HS RNA Amplification Kit
|
| Label |
biotin
|
| Label protocol |
Simultaneous cRNA synthesis and biotin labeling via 3' IVT protocol
|
| |
|
| Hybridization protocol |
10ug cRNA was used to make 250ul hyb cocktail, of which 200ul were injected into GeneChip X3P arrays and hybridized for 16 hours at 45C. Arrays were washed and stained using Affymetrix Fluidics Station 450 with user-prepared reagents.
|
| Scan protocol |
Microarrays were scanned using the GeneChip Scanner 3000 system from Affymetrix.
|
| Data processing |
Data were normalized using RMA and the Affymetrix Command Console module.
|
| |
|
| Submission date |
Oct 24, 2016 |
| Last update date |
Oct 19, 2017 |
| Contact name |
Hui Pan |
| E-mail(s) |
Hui.Pan@joslin.harvard.edu
|
| Organization name |
Joslin Diabetes Center
|
| Department |
Bioinformatics and Biostatistics Core
|
| Street address |
1 Joslin Place
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1352 |
| Series (1) |
| GSE89120 |
Evidence of stress in beta cells obtained with laser capture microdisssection from pancreases of brain-dead donor |
|
