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Sample GSM2359127 Query DataSets for GSM2359127
Status Public on Oct 17, 2017
Title Surgical7
Sample type RNA
Source name Surgical: Human Islets LCM
Organism Homo sapiens
Characteristics tissue: pancreas
cell type: Islets LCM
age: 77
gender: F
bmi: 26
Growth protocol LCM samples were obtained from selected beta cell rich autofluorescent areas of islets or from generalized non-selected areas LCM of islet tissue.
Extracted molecule total RNA
Extraction protocol PicoPure, Dnase treatment, RiboAmp HS RNA Amplification Kit
Label biotin
Label protocol Simultaneous cRNA synthesis and biotin labeling via 3' IVT protocol
Hybridization protocol 10ug cRNA was used to make 250ul hyb cocktail, of which 200ul were injected into GeneChip X3P arrays and hybridized for 16 hours at 45C. Arrays were washed and stained using Affymetrix Fluidics Station 450 with user-prepared reagents.
Scan protocol Microarrays were scanned using the GeneChip Scanner 3000 system from Affymetrix.
Data processing Data were normalized using RMA and the Affymetrix Command Console module.
Submission date Oct 24, 2016
Last update date Oct 19, 2017
Contact name Hui Pan
Organization name Joslin Diabetes Center
Department Bioinformatics and Biostatistics Core
Street address 1 Joslin Place
City Boston
ZIP/Postal code 02215
Country USA
Platform ID GPL1352
Series (1)
GSE89120 Evidence of stress in beta cells obtained with laser capture microdisssection from pancreases of brain-dead donor

Data table header descriptions
VALUE RMA signal intensity

Data table
1053_3p_at 3.920407
117_3p_at 4.599277
1494_3p_f_at 4.966049
1552275_3p_s_at 3.874722
1552281_3p_at 6.573513
1552296_3p_at 3.639529
1552302_3p_at 2.084762
1552311_3p_a_at 6.155409
1552321_3p_a_at 2.168726
1552340_3p_at 3.392272
1552343_3p_s_at 3.523145
1552354_3p_at 2.845752
1552476_3p_s_at 4.576727
1552480_3p_s_at 1.65467
1552504_3p_a_at 4.595189
1552518_3p_s_at 1.877563
1552528_3p_at 5.168121
1552535_3p_at 4.759674
1552566_3p_at 3.767241
1552575_3p_a_at 3.735815

Total number of rows: 61359

Table truncated, full table size 1712 Kbytes.

Supplementary file Size Download File type/resource
GSM2359127_Jos4349.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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