|
Status |
Public on Oct 23, 2017 |
Title |
PC9_6 Input THZ1 [lab: Hammerman-DFCI] |
Sample type |
SRA |
|
|
Source name |
Adenocarcinoma
|
Organism |
Homo sapiens |
Characteristics |
cell line: PC9 chip antibody: None young_id: 20160513_4692 tissue: Lung drug: THZ1 concentration: 100nM cell number: 50 million treatment duration: 48h
|
Treatment protocol |
Cells were treated with THZ1 (100 nM) for 48 hours prior to cross linking with formaldehyde.
|
Growth protocol |
PC9 cells were cultured in RPMI media, supplemented with 10% FBS, and penicillin/streptomycin/L-glutamine. Cells were cultured at 37 °C in a humidified chamber in the presence of 5% CO2.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified. Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies.
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Aligned using /usr/local/bin/bowtie with configuration -p 4 --best -k 2 -m 2 --sam -l 40 hg19 Supplementary_files_format_and_content: WIG files(s) represent counts of aligned reads within 50 bp bins with each read being extended 200 in the direction of alignment. Counts are in reads-per-million and floored at 0.1
|
|
|
Submission date |
Oct 24, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Richard A Young |
E-mail(s) |
young_computation@wi.mit.edu
|
Phone |
617-258-5219
|
Organization name |
Whitehead Institute for Biomedical Research
|
Lab |
Young Lab
|
Street address |
9 Cambridge Center
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL16791 |
Series (2) |
GSE89128 |
Suppression of adaptive responses to targeted cancer therapy by transcriptional repression [ChIP-seq] |
GSE89129 |
Suppression of adaptive responses to targeted cancer therapy by transcriptional repression |
|
Relations |
BioSample |
SAMN05938416 |
SRA |
SRX2267481 |