|
| Status |
Public on Nov 21, 2016 |
| Title |
Input DNA |
| Sample type |
SRA |
| |
|
| Source name |
Neural progenitor cells, input
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
cell type: ES-derived neural progenitor cells
chip antibody: none
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin was sheared using truChIP High Cell Chromatin Shearing Kit with Non-ionic Shearing Buffer (Covaris).
ChIP-seq libraries were constructed using 5500 SOLiD Fragment 48 Library Core Kit. Libraries were sequenced on the SOLiD 5500 platforms according to the manufacturer's instructions.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
AB 5500 Genetic Analyzer |
| |
|
| Data processing |
Sequencing reads were mapped against mm9 using Bowtie.
Tet3-enriched regions were identified by MACS peak-calling software (version 1.4.2).
Genome_build: mm9 (MGSCv37)
Supplementary_files_format_and_content: .bed files
|
| |
|
| Submission date |
Oct 30, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
ANJANA RAO |
| E-mail(s) |
arao@lji.org
|
| Organization name |
La Jolla Institute for Allergy and Immunology
|
| Street address |
9420 Athena Circle
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL16790 |
| Series (2) |
| GSE89315 |
Tet proteins influence the balance between neuroectoderm and cardiac mesoderm fate choice by inhibiting Wnt signaling |
| GSE89316 |
Tet proteins influence the balance between neuroectodermal and mesodermal fate choice by inhibiting Wnt signaling |
|
| Relations |
| BioSample |
SAMN05953630 |
| SRA |
SRX2310412 |
